qPCR & SNP Genotyping

LGC Biosearch Technologies offers a variety of probe and primer types for real-time qPCR, including dual-labeled BHQ® probes, BHQplus® probes, Molecular Beacons, and Scorpions® primers.

Dual-Labeled BHQ® Probes

View the selection of fluorophore-quencher combinations for dual-labeled probes available from LGC Biosearch. We synthesize high quality dual-labeled probes followed by HPLC purification to ensure every order meets purity requirements. Every oligo receives rigorous QC by electrospray mass spectroscopy before releasing for shipment.

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BHQnova Probes

The most difficult qPCR applications demand double-quenched probes for optimum performance. BHQnova™ probes have improved quenching efficiency compared to traditional end-labeled probes, while also improving signal release upon amplification. BHQnova is most advantageous in longer probe designs, typically those over 25 bases, to boost the signal-to-noise ratio by overcoming the upper limit on sequence length.

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BHQplus Probes

An advanced technology for qPCR and SNP genotyping, the BHQplus® probe is a compact oligo fortified with our signature duplex stabilizing chemistry to elevate the melting temperature and enhance target specificity. BHQplus probes are the optimal choice for marker-assisted selection in the agricultural industry, and to research the polymorphisms that may influence human health.

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Molecular Beacons

Molecular Beacons have short complementary sequences that fold into a stem-loop structure. This hairpin conformation positions the fluorophore and quencher very close together in space, for remarkably efficient quenching. LGC Biosearch currently offers Molecular Beacons labeled with either a Black Hole Quencher® or a DABCYL dye. These quenchers are paired with a variety of fluorescent reporters.

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Scorpions Primers

Scorpions® primers combine the probe and primer into one oligo to accomplish a uni-molecular signaling mechanism. Enzymatic hydrolysis of the probe during PCR cycling is not required for signal release. View LGC Biosearch’s selection of Scorpions primers.

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Calibration Dyes

Do you intend to multiplex qPCR assays using FAM, CAL Fluor® and/or Quasar® dyes? Remember to optimize your instrument's performance by using the calibration standards available from LGC Biosearch.

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SuperROX® Reference Dye

SuperROX is a highly stable passive reference dye that is formulated to significantly reduce "ROX drop." It can be used when a water soluble version of ROX is required as a passive reference to normalize fluorescent signals in real-time PCR.

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ValuPanel Reagents

ValuPanel Reagents are composed of pre-designed probes and primers that are intended to resolve the genetic signatures to of different pathogen strains. They are for Research Use Only (RUO), not for use in diagnostic procedures.

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LGC Biosearch Technologies offers a variety of probe and primer formats for SNP genotyping and gene expression analysis with real-time qPCR. As inventors of the Black Hole Quencher® (BHQ®) dyes, every probe format is available with a BHQ dye for versatile quenching. In addition, each oligo may be labeled with a variety of fluorophores that span the spectrum, such as FAM, TET, HEX, JOE, CAL Fluor®, and Quasar® dyes. View our Dye Selection Chart or Spectral Overlay Tool for Multiplexed qPCR to see a list of dyes offered at LGC Biosearch. Other modifications are also available for advanced probe designs. 

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