qPCR & SNP Genotyping

LGC Biosearch Technologies offers a variety of probe and primer types for real-time qPCR, including dual-labeled BHQ® probes, BHQplus® probes, Molecular Beacons, and Scorpions® primers.

Dual-Labeled BHQ® Probes

View the selection of fluorophore-quencher combinations for dual-labeled probes available from LGC Biosearch. We synthesize high quality dual-labeled probes followed by HPLC purification to ensure every order meets purity requirements. Every oligo receives rigorous QC by electrospray mass spectroscopy before releasing for shipment.

learn more

BHQnova Probes

The most difficult qPCR applications demand double-quenched probes for optimum performance. BHQnova™ probes have improved quenching efficiency compared to traditional end-labeled probes, while also improving signal release upon amplification. BHQnova is most advantageous in longer probe designs, typically those over 25 bases, to boost the signal-to-noise ratio by overcoming the upper limit on sequence length.

learn more

BHQplus Probes

An advanced technology for qPCR and SNP genotyping, the BHQplus® probe is a compact oligo fortified with our signature duplex stabilizing chemistry to elevate the melting temperature and enhance target specificity. BHQplus probes are the optimal choice for marker-assisted selection in the agricultural industry, and to research the polymorphisms that may influence human health.

learn more

BHQ probes master mix

For high-precision genotyping, pair BHQ Probe Master Mix with BHQ or BHQplus probes for a high-fidelity, low background SNP genotyping assay that yields clear results minus the challenges associated with ambiguous data interpretation.

learn more

Molecular Beacons

Molecular Beacons have short complementary sequences that fold into a stem-loop structure. This hairpin conformation positions the fluorophore and quencher very close together in space, for remarkably efficient quenching. LGC Biosearch currently offers Molecular Beacons labeled with either a Black Hole Quencher® or a DABCYL dye. These quenchers are paired with a variety of fluorescent reporters.

learn more

Scorpions Primers

Scorpions® primers combine the probe and primer into one oligo to accomplish a uni-molecular signaling mechanism. Enzymatic hydrolysis of the probe during PCR cycling is not required for signal release. View LGC Biosearch’s selection of Scorpions primers.

learn more

Calibration Dyes

Do you intend to multiplex qPCR assays using FAM, CAL Fluor® and/or Quasar® dyes? Remember to optimize your instrument's performance by using the calibration standards available from LGC Biosearch.

learn more

SuperROX® Reference Dye

SuperROX is a highly stable passive reference dye that is formulated to significantly reduce "ROX drop." It can be used when a water soluble version of ROX is required as a passive reference to normalize fluorescent signals in real-time PCR.

learn more

ValuPanel Reagents

ValuPanel Reagents are composed of pre-designed probes and primers that are intended to resolve the genetic signatures to of different pathogen strains. They are for Research Use Only (RUO), not for use in diagnostic procedures.

learn more

LGC Biosearch Technologies offers a variety of probe and primer formats for SNP genotyping and gene expression analysis with real-time qPCR. As inventors of the Black Hole Quencher® (BHQ®) dyes, every probe format is available with a BHQ dye for versatile quenching. In addition, each oligo may be labeled with a variety of fluorophores that span the spectrum, such as FAM, TET, HEX, JOE, CAL Fluor®, and Quasar® dyes. View our Dye Selection Chart or Spectral Overlay Tool for Multiplexed qPCR to see a list of dyes offered at LGC Biosearch. Other modifications are also available for advanced probe designs. 

Learn more: