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Stellaris Universal Controls

Universal controls for Stellaris® RNA FISH are convenient and easy-to-use positive controls for smooth adoption of the Stellaris technology. The Stellaris universal controls hybridise to the polyA tail of both immature and mature mRNAs to produce a well-known fluorescence pattern consistent with their cytoplasmic and nuclear distribution.

Key Benefits

  • A reliable and inexpensive positive control for validation and optimization of RNA FISH experiment setup

  • Hybridise to the polyA tail of both immature and mature RNAs

  • Produce a well-known fluorescence pattern consistent with broad cytoplasmic distribution of mature RNAs, lncRNAs, and pre-RNAs in nuclear speckles

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Product information: One vial of Stellaris universal controls contains 1 nmol of T30 oligo labeled with a fluorophore. These controls arrive lyophilized and ready to use. Delivered Amount: 1 nmol of singly labeled 30-mer of thymidines (T30)

Turnaround time: Stellaris universal positive controls ship the same day for orders placed before 2:00 PM (PST)

Protocols: Please visit our Stellaris RNA FISH Protocols page

Absorption and emission spectral charts: Please visit our Calibration dyes page.

Figure 1. Stellaris universal control.
The T30 single labeled oligo is compatible with the Stellaris protocols. The T30-oligo reveals the intracellular polyA distribution in a “one-pot shot” for easy cell boundary identification for single molecule RNA detection and counting. Shown here is a combination-experiment with mouse C2C12 cells. The polyA RNA was probed with a Quasar 570 labeled T30 oligo (left and right panel; green cytoplasm and nculear speckles), mouse Tbp mRNAs with a probe set labeled with Quasar 670 (middle and right panel; red cytoplasmic spots), and nuclear DNA stained with DAPI (middle and right panel; blue nucleus).

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