Stellaris® RNA FISH Buffers contain proprietary additives to enhance detection with Stellaris RNA FISH probe sets, particularly for applications that typically exhibit more pronounced background fluorescence.
Before getting started with your first experiments, please visit our Stellaris RNA FISH information page and download our Getting Started Guide. You can also learn more about the Stellaris RNA FISH technology by downloading our white paper. Furthermore, The BiosearchTech Blog contains the most up-to-date tips and tricks for running Stellaris RNA FISH experiments.
Stellaris RNA FISH Buffers outperform Conventional RNA FISH buffers
A direct comparison showing background reduction by using Stellaris RNA FISH Buffers (Cat#: SMF-HB1-10, SMF-WA1-60, SMF-WB1-20). Following the adherent cell protocol, formalin fixed human cells were probed with Human POLR2A (Cat# VSMF-2293-5). Left Panel: RNA FISH performed with Stellaris RNA FISH Buffers. Right Panel: RNA FISH performed with Conventional RNA FISH buffer recipes. Note: Results may vary according to original probe set performance and sample type.