Stellaris™ RNA FISH is a RNA visualisation method that allows simultaneous detection, localisation, and quantification of individual mRNA molecules at the cellular level in fixed samples. This novel RNA FISH technology represents a fast and easy-to-use method to achieve conclusive results through compelling images of RNA expression.

Stellaris RNA FISH Buffers

Stellaris Buffers are ideal for performing Stellaris RNA FISH on cells and tissue, as well as other sample types. The buffers contain proprietary additives to enhance signal and reduce background in assays that may typically exhibit more pronounced background fluorescence

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Stellaris FISH (fluorescence in situ hybridisation) is a RNA visualisation method that allows simultaneous detection, localization, and quantification of individual RNA molecules at the sub-cellular level in fixed samples using widefield fluorescence microscopy. A set of Stellaris FISH Probes comprises multiple oligonucleotides with different sequences each with a fluorescent label that collectively bind along the same target transcript to produce a punctate signal.

The Stellaris FISH technology follows a simple protocol, does not use exotic reagents, is inexpensive and platform-independent, offers same-day results, and is versatile toward many sample types and applications. Scientists may even label Stellaris FISH probes with a number of various dyes to allow for multiplex detection of different RNA targets simultaneously. Finally, scientists can address the stochastic nature of gene expression and visualise RNA through direct detection without isolation, purification, and amplification.

Before getting started with your experiments, please download our Getting Started Guide. You can also learn more about the Stellaris RNA FISH technology by downloading our white paper or by visiting our RNA FISH applications page. Furthermore, The BiosearchTech Blog contains the most up-to-date tips and tricks for running Stellaris experiments.

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