Stellaris Custom RNA FISH probe sets can be designed against almost any RNA target to allow for visualization, localization, and quantification of individual mRNA transcripts.learn more
Stellaris ShipReady RNA FISH Probe sets provide convenient, ready-to-ship positive controls for reference and long non-coding RNA targets.learn more
Stellaris DesignReady probe sets are professionally designed, and go through rigorous bioinformatic analysis to ensure specificity. Stellaris DesignReady Probe Sets are made-to-order.learn more
Stellaris Buffers are ideal for performing Stellaris RNA FISH on cells and tissue, as well as other sample types. The buffers contain proprietary additives to enhance signal and reduce background in assays that may typically exhibit more pronounced background fluorescencelearn more
ChIRP is a rapid technique to discover RNA-associated DNA sequences and map genomic binding sites of chromatin associated long noncoding RNAs (lncRNAs) with high sensitivity and low background.learn more
View Stellaris protocols, educational materials, citation center, image gallery, webinars, tech articles, FAQ, and more.learn more
Stellaris FISH (fluorescence in situ hybridisation) is a RNA visualisation method that allows simultaneous detection, localization, and quantification of individual RNA molecules at the sub-cellular level in fixed samples using widefield fluorescence microscopy. A set of Stellaris FISH Probes comprises multiple oligonucleotides with different sequences each with a fluorescent label that collectively bind along the same target transcript to produce a punctate signal.
The Stellaris FISH technology follows a simple protocol, does not use exotic reagents, is inexpensive and platform-independent, offers same-day results, and is versatile toward many sample types and applications. Scientists may even label Stellaris FISH probes with a number of various dyes to allow for multiplex detection of different RNA targets simultaneously. Finally, scientists can address the stochastic nature of gene expression and visualise RNA through direct detection without isolation, purification, and amplification.
Before getting started with your experiments, please download our Getting Started Guide. You can also learn more about the Stellaris RNA FISH technology by downloading our white paper or by visiting our RNA FISH applications page. Furthermore, The BiosearchTech Blog contains the most up-to-date tips and tricks for running Stellaris experiments.