Focusing on the finer points of the SARS-CoV-2 testing workflow: false positives and false negatives
17 December, 2020 / Understanding misleading COVID-19 test results
Like any diagnostic test, SARS-CoV-2 molecular diagnostic assays are affected by the appearance of false positives and false negative results. In this episode, we speak with qPCR assay, workflow, and measurement experts Jim Huggett, Tania Nolan, and James Grayson about the causes of false positives and false negatives and how to mitigate these misleading events. Along the way we also touch on limits-of-detection, the role of workflow software in supporting the production of reliable results, and important aspects of the qPCR assay that clinicians and assay developers should keep in mind.