MasterAmp™ Tth DNA Polymerase is a recombinant DNA enzyme from Thermus thermophilus that has both DNA-dependent and RNA-dependent DNA polymerase activities up to ~95 °C. MasterAmp Tth DNA Polymerase is highly purified using a proprietary procedure that virtually eliminates contaminating bacterial DNA.

Applications

• PCR amplification of DNA and one-step RT-PCR of RNA.
• PCR amplification of RNA and DNA templates having a high degree of secondary structure.

Optimisation of [Mg ²⁺] and [Mn ²⁺]: Magnesium and manganese ion concentrations should be optimised for DNA synthesis. The optimal concentration of each metal cation is highly dependent on the dNTP concentration and on the template, primers, and protocol used. For many templates, the optimal concentration of magnesium ions using MasterAmp Tth DNA Polymerase is approximately 1.5 mM if the concentration of each dNTP in the reaction is 0.2 mM. If used with 1.5 mM Mg ²⁺, the optimal concentration of Mn ²⁺ for RNA-dependent DNA synthesis is approximately 0.5 mM for many templates. For an RT-PCR reaction, we recommend 3.0 mM Mg ²⁺ and 0.5 mM Mn ²⁺ for most templates. Alternatively, we recommend the MasterAmp PCR Optimisation Kit with ammonium sulfate for rapid PCR optimisation.