Large or Difficult Cloning
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Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
Large or Difficult Cloning
Protein Expression
General Cloning and Library Construction
Protein Expression
Large or Difficult Cloning
Format
Format
Size
E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™ and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required. These cells provide solutions for a wide range of applications at economical prices.
E. cloni 5-alpha Chemically Competent Cells >1 × 10 8 cfu/µg DNA
Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.
Table 1. E. cloni Competent Cells for Cloning
E. cloni Cell Lines |
Transformation Efficiency |
Cloning Methylated DNA |
BAC, Cosmid Cloning |
Blue/White Screening |
5-alpha Chemically Competent |
>1 × 10 8 cfu/µg DNA |
NO |
NO |
YES |
E. cloni 5-alpha: fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
Efficiencies
E. cloni 5-alpha Chemically Competent Cells >1 × 108 cfu/µg DNA
Large or Difficult Cloning
Recovery Media
Large or Difficult Cloning
Size
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