Skip to content Skip to navigation menu
New Site Launched: Looking for oligos? Visit our new oligo and Stellaris dedicated platform at oligos.biosearchtech.com.
cancel
  1. Home
  2. Competent cells for transformation
  3. Phage Display Library Construction
  4. SS320 (MC1061 F') Electrocompetent Cells

SS320 (MC1061 F') Electrocompetent Cells

SS320 (MC1061 F') Electrocompetent Cells

Product Details

Show Hide

Competent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

SS320 (MC1061 F') Electrocompetent Cells

Construct larger, more complex phage display libraries with highest efficiency cells. SS320 is a non-amber suppressor strain used for phage display.

Key features

Show Hide
  • Perfect for antibody phage display library creation
  • Create larger libraries; speed discovery
  • Bulk custom dispensing available with all of our phage display cells
Select a Size
TBD
Add to basket to request a quote

Similar Products

TG1 Electrocompetent Cells

Phage Display Library Construction

TG1 Electrocompetent Cells

Construct larger, more complex phage display libraries with highest efficiency cells. This amber suppressor strain can be used for phage display and protein expression.
View
OverExpress Electrocompetent Cells

Protein Expression

OverExpress Electrocompetent Cells

Express difficult or toxic proteins using standard E. coli T7 expression vectors using these unique strains. Also available in chemically competent format.
View
Added to basket

Product information

SS320 Electrocompetent Cells. A non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells have the highest available transformation efficiency of any strain used for phage display.

Transformation efficiency of Biosearch Technologies electrocompetent bacterial cells for phage display compared to competitors

Figure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.

Table 1. Competent Cells for Phage Display libraries


Cell Lines

Transformation Efficiency
(cfu/µg pUC DNA)

Cloning Methylated DNA

BAC, Cosmid Cloning

Blue/White Screening

TG1 Electrocompetent

>4 × 1010

YES

NO

YES
IPTG induction required

SS320 (MC1061 F') Electrocompetent

>4× 1010

YES

NO

YES
IPTG induction required

MC1061 F-Electrocompetent

>4× 1010

YES

NO

YES
IPTG induction required

ER2738 Electrocompetent

>2 × 1010

YES

NO

YES
IPTG induction required

Genotype

  • TG1: [F' traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)
  • SS320 (MC1061F'): F'[proAB lacIqZ ΔM15 Tn10 (TetR)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1
  • ER2738: [F'proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5
  • MC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1

SDS

Manuals and user guides

Product information sheets

Application notes

You may also be interested in...

Expression Recovery Medium

Recovery Media

Expression Recovery Medium

Optimised formulation for ensuring stability and high-efficiency transformation of protein expression clones.
ITEM ID
80030-1
View
TransforMax EPI300

Size

TransforMax EPI300

Clone large inserts in standard plasmids, and control copy number of CopyControl vectors. EPI300 Electrocompetent cells are recommended for Gibson Assembly® reactions.
View
Added to basket

Access support

Need some support with placing an order, setting up an account, or finding the right protocol?

Contact us