sbeadex™ magnetic beads enable automated high-throughput extraction and purification of high-quality nucleic acids from easy to challenging sample types.

An optimised chemistry with a novel two-step binding mechanism allows nucleic acids to bind more specifically to RNA and DNA purification beads. Final washes with pure water deliver nucleic acids of high yield, purity and quality that can be used in many downstream applications such as PCR/qPCR/RT-qPCR or NGS. The sbeadex magnetic bead-based DNA and RNA purification procedure is fast, reliable and compatible with many robotic platforms, including our oKtopure™.

See how sbeadex nucleic acid purification works

Applications of sbeadex nucleic acid purification kits

  • DNA and RNA purification for many downstream applications such as PCR/qPCR, RT-PCR or NGS
  • Applicable to a wide range of sample types used in basic or biopharmaceutical research as well as molecular diagnostics and agricultural screening and testing
    • sbeadex plant: Various tissues (including leaf, seed, bark, pulp and root) from a variety of plant species
    • sbeadex livestock: Various sample material (e.g. blood, tissue, hair, semen, saliva, ear punch) from a variety of animal species
    • sbeadex blood: Whole blood, EDTA blood, heparin blood, citrate blood, buffy coats
    • sbeadex tissue: Cultured cells and tissues (e.g. skin, lung, muscle, spleen, mouse tails, ear punches)
    • sbeadex pathogen: Viral and bacterial pathogens (e.g. SARS-CoV-2, Influenza, Legionella, Mycoplasma)
    • sbeadex plasmid: Bacterial cultures
    • sbeadex forensic: Broad range of forensic samples, including blood stains, hair follicles, chewing gum and clothing
    • sbeadex PCR clean-up: Purify PCR products by removing primers, unincorporated nucleotides, enzymes and other unwanted contaminants
    • sbeadex SAB: Use for DNA clean-up and DNA fragment size selection during NGS library prep and PCR methods

Key benefits of sbeadex technology

  • Highly purified nucleic acids suitable for many downstream applications 
  • Fast, convenient and reproducible protocols that are easily adaptable to high-throughput automation
  • No organic extraction, alcohol precipitation, alcohol washes or centrifugation steps
  • Water-based wash buffers
  • No potential PCR inhibitors (e.g. organic solvents, ethanol or salts) in wash buffers and eluate
  • Highly stable, ready-to-use kits
  • Compatible with most popular robotic platforms (e.g. KingFisher, Hamilton, Tecan, Beckman-Coulter, or our high-throughput oKtopure instrument)

Partner with us to customise sbeadex technology for your specific requirements. We offer highly flexible batch sizes and tailored protocols (e.g. modified lysis or elution steps) as well as customised and bulk sized kits. Contact us to discuss your project.

DNA/RNA preparation protocol with sbeadex magnetic beads

sbeadex magnetic beads allow efficient and high-quality purification of small and large DNA and RNA fragments within 45 minutes without centrifugation steps. The beads are double coated, requiring a unique dual binding protocol. This protocol allows elution of ultrapure nucleic acids into water and significantly reduces solvent carryover that might affect downstream applications.

sbeadex workflow order

The particles used in the sbeadex™ and mag™ kits are modified superparamagnetic particles, which allow efficient and high quality purification of small and large DNA fragments. These allow efficient and high quality purification of small and large nucleic acid fragments of several sample materials. The beads are attracted by strong permanent magnets, but they do not become permanent magnets.

Parameters of the magnetic particles:

Colour Dark brown
Size <53 µm, 80% 5-10 µm
Shape irregular
Specific weight 3.0-3.5 g/10 mL dry particles
Maximum capacity 2 µg DNA/mg particles
Storage/stability >2 years as dry powder at room temperature (normally the beads are delivered as suspension which is stable approx. 6 months)

LGC Biosearch Technologies developed standard protocols on a broad variety of robotic platforms for extractions of all major plant species, plant samples and other sample matrices. As protocols on the KingFisher platform can easily be transferred and installed, we provide application notes based on our sbeadex standard chemistry. For all other robotic platforms and sample material not mentioned, please contact us. The extractions team is very experienced with a wide range of sample matrices, having worked with all kinds of samples in our DNA extraction service laboratories. We are able to send you DNA extraction protocols for many sample types that are not mentioned here.

sbeadex SDS by kit

 

All sbeadex and mag SDS

5 x Enrichment Buffer BLL - 100 kB

Binding additive TN EN - 46.3 kB

Binding Buffer BL - 181 kB

Binding buffer PN 2 - 202 kB

Binding buffer PN FN (concentrate) - 77.0 kB

Binding Buffer PN FN PCR - 200 kB

Binding Buffer SB - 301 kB

Binding buffer SB (concentrate) - 182 kB

Buffer EB BL - 37.0 kB

debris capture beads - 37.1 kB

Elutions Buffer 4xAMP for EU - 43.8 kB

Elutions Buffer AMP - 54.1 kB

Elutions Buffer BL BLM BLm PN FN PLN - 37.4 kB

Lysis Buffer BL - 59.3 kB

Lysis Buffer BLm BLM - 89.1 kB

Lysis Buffer BLQ - 48.5 kB

Lysis Buffer BNM Binding Buffer TN - 76.9 kB

Lysis Buffer EX1 - 179 kB

Lysis Buffer EX2 - 180 kB

Lysis Buffer Hair - 48.6 kB

Lysis Buffer NA - 182 kB

Lysis Buffer P Lysis Buffer PN - 193 kB

Lysis buffer PLN - 153 kB

Lysis Buffer PVP - 179 kB

Lysis buffer PVP + Lysis buffer SB - 183 kB

Lysis Buffer SB - 182 kB

Lysis Buffer TN - 48.5 kB

Lysis- Wash Buffer C1 - 165 kB

mag particle suspension BLM - 131 kB

mag particle suspension maxi - 37.1 kB

Neutralisation buffer PLN - 118 kB

PK Solution Protease Solution - 114 kB

Resuspension buffer PLN - 37.1 kB

RNase A - 47.3 kB

Sample storage buffer - 193 kB

sbeadex SL binding mix - 38.7 kB

sbeadex⌐ particle suspension - 37.0 kB

sbeadex⌐ particle suspension + EDTA - 43.5 kB

Wash Buffer A1 - 154 kB

Wash Buffer A1 (concentrate) - 37.1 kB

Wash Buffer BL1 - 36.9 kB

Wash Buffer BL2 BLM2 BLm2 - 37.2 kB

Wash Buffer BLm 1 BLM 1 - 163 kB

Wash Buffer BN1 - 226 kB

Wash Buffer BN1 (concentrate) - 90.9 kB

Wash Buffer PB - 196 kB

Wash Buffer PN1 FN1 TN1 - 200 kB

Wash Buffer PN1 FN1 TN1 (concentrate) - 77.0 kB

Wash Buffer PN2 FN2 TN2 BLN PLN for EU - 38.8 kB

Product name Item name Number of purifications per kit  
sbeadex mini plant kit (20-30 mg starting material) NAP41601 96 Order now
sbeadex mini plant kit (20-30 mg starting material) NAP41610 960 Order now
sbeadex maxi plant kit (80-100 mg starting material) NAP41602 96 Order now
sbeadex maxi plant kit (80-100 mg starting material) NAP41620 960 Order now
sbeadex maxi plant kit (80-100 mg starting material) NAP40-002-03 5000 Order now
sbeadex tissue kit (DNA extraction from 20-30 mg tissue) NAP41405 96 Order now
sbeadex tissue kit (DNA extraction from 20-30 mg tissue) NAP41450 960 Order now
sbeadex blood kit NAP44401 96 Order now
sbeadex blood kit NAP44410 960 Order now
sbeadex blood kit NAP44100 5000 Order now
sbeadex Pathogen Nucleic Acid Purification Kit NAP-40-024-01 96 Order now
sbeadex Pathogen Nucleic Acid Purification Kit NAP-40-024-02 960 Order now
sbeadex Pathogen Nucleic Acid Purification Kit NAP-40-024-03 5000 Order now
sbeadex livestock kit NAP44701 96 Order now
sbeadex livestock kit NAP44702 960 Order now
sbeadex livestock kit NAP44703 5000 Order now
sbeadex plasmid kit NAP41301 96 Order now
sbeadex plasmid kit NAP41310 960 Order now
sbeadex forensic kit NAP41501 96 Order now
sbeadex forensic kit NAP41510 960 Order now
sbeadex PCR clean-up kit NAP41701 96 Order now
sbeadex PCR clean-up kit NAP41710 960 Order now
Debris capture beads NAP41151 10 mL Order now
Debris capture beads NAP41152 100 mL Order now
sbeadex SAB kit (sequencing application beads) NAP45001 5 mL Order now
sbeadex SAB kit (sequencing application beads) NAP45002 20 mL Order now

Notes:
Some kits are available with “no dangerous goods” labels or without proteinase K. We also provide customised solutions as well as cost-effective bulk sizes. Please contact us to discuss your specific requirements.

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QuickExtract Plant DNA Extraction Solution View product
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MasterPure Yeast DNA Purification Kit View product
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oKtopure™ automated DNA extraction system View product

Please find more products for genomics applications (e.g. PCR/qPCR, RT-PCR, gene cloning & expression, library construction, in vitro transcription, NGS) by visiting our PCR Kits & Reagents site or General Products for Molecular Biology. Please note that we also provide Instruments and consumables, NGS kits,  as well as Custom oligonucleotides.

For Research Use Only. Not for use in diagnostic procedures.