Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube) or FIVERs (5 transformations per tube), as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
Low Endoxin Cells
Large or Difficult Cloning
Phage Display Library Construction
Phage Display Library Construction
Protein Expression
Phage Display Library Construction
Competent cells for transformation
Phage Display Library Construction
We offer two bacterial strains designed for BAC library construction or transformation of large constructs:
BAC-Optimised Replicator™ v2.0 Electrocompetent Cells (> 1 × 1010 cfu/µg pKanR DNA). These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimised Replicator Cells are components of Biosearch Technologies Cloning Kits
E. cloni 10G BAC-Optimised Electrocompetent Cells (> 1 × 107 cfu/µg 150 kb BAC DNA; > 1 × 1010 cfu/µg pUC DNA). The first competent cells developed specifically for large DNA cloning. Manufactured using a novel procedure that maximises transformation efficiencies with very large inserts, 10G BAC-Optimised Cells are an excellent choice for BAC library construction and large construct transformation.
Table 1. E. cloni Competent Cells for BAC or Large Fragment Cloning
E. cloni Cell Lines |
Transformation Efficiency |
Cloning Methylated DNA |
BAC, Cosmid Cloning |
Blue/White Screening |
|
10G BAC-Optimised Electrocompetent |
>1 × 1010 |
YES |
YES |
YES |
|
BAC-Optimised Replicator v2.0 Electrocompetent |
>1 × 1010 |
YES |
YES |
YES |
General Cloning and Library Construction
Protein Expression
Protein Expression
General Cloning and Library Construction
Large or Difficult Cloning
Format
Format
Size
Size
Replicator v2.0: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galUgalK rpsL nupG (attL araC-PBAD-trfA250 bla attR) λ-. Note that Replicator v2.0 cells are AmpR (bla gene) (StrR)
E. cloni 10G BAC-Optimized: F - mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupG λ- tonA (StrR)
Large or Difficult Cloning
Recovery Media
Large or Difficult Cloning
Size
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Contact us| Europe, Middle East, and Africa | |
|---|---|
| UK | +44 1992 470 757 |
| Germany | +49 30 5304 2200 |
| North America, Latin America | |
| Wisconsin, USA | +1 888 575 9695 |
| Asia Pacific | |
| China | +8621-22509000 |
| Singapore | +65 6734 4800 |
