Competent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
Low Endoxin Cells
Large or Difficult Cloning
Phage Display Library Construction
Large or Difficult Cloning
Protein Expression
Phage Display Library Construction
Competent cells for transformation
Phage Display Library Construction
MC1061 F- Electrocompetent Cells. A non-amber suppressor strain, identical to SS320 except that it lacks the F′episome. Highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for general cloning or Phage Display. Note that as an F- strain, it cannot be used for re-infection by filamentous phage. This strain not included in the sample kit.
Figure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.
Table 1. Competent Cells for Phage Display libraries
Cell Lines |
Transformation Efficiency |
Cloning Methylated DNA |
BAC, Cosmid Cloning |
Blue/White Screening |
|
TG1 Electrocompetent |
>4 × 1010 |
YES |
NO |
YES |
|
SS320 (MC1061 F') Electrocompetent |
>4× 1010 |
YES |
NO |
YES |
|
MC1061 F- Electrocompetent |
>4× 1010 |
YES |
NO |
YES |
|
ER2738 Electrocompetent |
>2 × 1010 |
YES |
NO |
YES |
General Cloning and Library Construction
Protein Expression
Protein Expression
General Cloning and Library Construction
Large or Difficult Cloning
Format
Format
Size
Size
Genotype
Large or Difficult Cloning
Recovery Media
Large or Difficult Cloning
Size
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