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Rec J Exonuclease
Magnesium-dependent exonuclease for 5' to 3' digestion of single-stranded DNA.
Key features
Show- Specific: Digests ssDNA in the 5' to 3' direction
- Heat Inactivated: Heat at 65 °C for 20 minutes to kill enzyme activity
- Flexible: Use to remove primers from completed PCR or to degrade single-stranded linear DNA in dsDNA and plasmid preps
Size: 250 units
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Product information
Rec J Exonuclease, derived from E. coli, catalyses degradation of ssDNA in a 5´ to 3´ direction. Its activity is dependent on Mg2+. Rec J Exonuclease can be heat-inactivated by incubation at 65 °C for 20 minutes.
Applications
- Removal of primers from completed PCR.
- Degradation of single-stranded linear DNA in dsDNA and plasmid preps.
Unit Definition: One unit of Rec J Exonuclease catalyses the release of 1 nmol of acid-soluble nucleotides from activated single-stranded calf thymus DNA in 30 minutes at 37 °C under standard assay conditions.
Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.1 M NaCl, and 0.1% Triton® X-100.
10X Reaction Buffer: 330 mM Tris-acetate (pH 7.5), 660 mM potassium acetate, 100 mM magnesium, acetate, and 5.0 mM DTT.
Quality Control: Rec J Exonuclease is free of detectable RNase and contaminating DNA endonuclease activities.
SDS
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