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Thermostable Pyrophosphatase

Thermostable Pyrophosphatase

Product Details

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Contains either 1 x 0.25 mL vial (300TAPP-1) or 4 x 0.25 mL vials (300TAPP-2) of Thermostable Pyrophosphatase, 2 U/µL.

Research Use Only

Thermostable Pyrophosphatase

Selectively degrades inhibitory inorganic pyrophosphate in DNA synthesis reactions

Key features

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  • Supplied at 2 U/µL
  • Stable at temperatures > 95 °C
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Product information

Thermostable pyrophosphatase is a recombinant, heat-tolerant (>95°C) acidic pyrophosphatase from a thermophilic archaebacterium. This enzyme selectively hydrolyzes inorganic pyrophosphate into two orthophosphate ions under standard buffered conditions (pH 6-9) but will not degrade other molecules containing phosphodiester bonds (e.g. nucleotide triphosphates). Inorganic pyrophosphatases can be added to DNA synthesis and amplification reactions to improve reaction kinetics.

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