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5'-DMT-Amino Modifier C12 CE-Phosphoramidite
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5'-DMT-Amino Modifier C12 CE-Phosphoramidite
CAS No.:1027512-19-1
An amino-modifier phosphoramidite with an acid-labile DMT protecting group.
Key features
Show- Incorporates primary amine to 5' end of an oligonucleotide, for subsequent conjugation.
- Acid-labile DMT protection
- C12 spacer useful in e.g. affinity chromatography, where the oligo must be sufficiently distanced from the surface, and in some cases labelling with fluorescent tags, where close interaction may lead to partially quenched fluorescence.
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Product information
The installation of an amino-modifier at the 5'-terminus of an oligonucleotide provides, via amide bond formation, a handle for the attachment of a variety of chemical species. If purification of the amine-modified oligonucleotide is desired, it is common to employ an MMT group on the amine (see eg. LK2123 and LK2133). The lipophilicity of the MMT group aids in reversed-phase purification techniques and may also be useful for assaying the coupling yield. For applications where acid sensitivity is an issue, we offer the more acid-labile DMT-protected 5'-Amino-modifier-C12 phosphoramidte. Amine-modified oligonucleotides have been synthesized using closely related DMT-bearing amino-modifier phosphoramidites.(1) For coupling, ese standard protocols; extended coupling times are not required.
Ref:
- (a) Sinha, N. D.; Cook, R. M. Nucleic Acids Res.1988, 16, 2659-2669. (b) Guar, R. K. Nucleosides & Nucleotides1991, 10, 895-909.
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