Installs an amine at the 5'-terminus of oligonucleotides enabling fluorous affinity purification.⁽¹⁾ For applications requiring the purification of 5'-amine-modified oligonucleotides, trityl-on purification is an option, typically involving a monomethoxytrityl (MMT) group. In cases where the separation of the trityl-on oligonucleotides from failure sequences and other non-trityl-bearing materials is more difficult, FL1500 is a useful option. It employs a fluorous version of the MMT group, i.e., a fluorous monomethoxytrityl (FMMT) group. This phosphoramidite behaves similarly to the MMT versions during synthesis, but the final FMMT-bearing oligonucleotide is more strongly retained on fluorous adsorbents or reversed-phase adsorbents, providing greater selectivity than observed for MMT-bearing oligos.

Ref:

1. Pearson, W. H.; Berry, D. A.; Stoy, P.; Jung, K.-Y.; Sercel, A. D. J. Org. Chem. 2005, 70, 7114-7122.