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5'-DMT-dU-Suc-CPG (5'-DMT-deoxyUridine CPG)
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5'-DMT-dU-Suc-CPG (5'-DMT-deoxyUridine CPG)
CPG used to incorporate a deoxyuridine base into the 3' end of an oligonucleotide.
Key features
Show- Used to induce mutagenic effects.
- The enzyme uracil-N-glycosylase (UNG) can specifically remove uracil to create abasic sites at the deoxyuridine positions. This property is used to generate site-specific strand breaks in the oligonucleotide.
- CPG has aminopropyl-succinyl linker.
- 1000 Å CPG suitable for highly modified oligonucleotides (> 20mers).
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Product information
Incorporation of a deoxyuridine base within a DNA sequence can be used to induce mutagenic effects. The enzyme uracil-N-glycosylase (UNG) can specifically remove uracil to create abasic sites at the deoxyuridine positions. This property is used to generate site-specific strand breaks in the oligonucleotide. We provide both dU phosphoramidite and CPGs.
Properties:
- Appearance: white powder
Product usage:
- Cleavage conditions: Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.
- Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C. Image of cleaved and deprotected structure:
- The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 290.17
Storage and handling:
- Shipping conditions: Ambient
- Storage conditions: +2 to +8 °C
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