N4-Etheno-dC CEP (BA 0391), enables the incorporation of a fluorescent pyrimidine nucleoside analog into oligonucleotides for the investigation of nucleic acid interactions. Due to the similarity in structure between dC and 3, N4-Etheno-dC, the modified nucleoside can be incorporated into DNA with retention of DNA structure and nucleobase recognition.43, N4-Ethenocytosine is reported to be a highly mutagenic DNA adduct that is recognized and excised by the double-stranded uracil DNAglycolase in E. Coli, and by human thymine-DNA glycolase proteins in vitro. The miscoding potential in reactions catalyzed by mammalian DNA polymerases for3, N4-etheno2'-deoxycytidine has also been reported. Both BA 0403and BA 0391 can be efficiently incorporated into oligonucleotides using standard conditions and taking care to avoid prolonged exposure to ammonium hydroxide.

3, N4-Etheno-dC-CE Phosphoramidite is a fluorescent pyrimidine nucleoside analogue that is useful for the investigation of nucleic acid interactions. Due to the similarity in structure between dC and 3, N4-Etheno-dC, the modified nucleoside can be incorporated into DNA with retention of DNA structure and nucleobase recognition.⁽¹⁾

Ref:

1. Ming, X.; Seela, F. Chem. Eur. J. 2012, 18, 9590-9600 and references therein.