Contents: Red Cell Lysis Solution, Tissue and Cell Lysis Solution, MPC Protein Precipitation Reagent, RNase A, TE Buffer.
Size: 1 kit
DNA and RNA Purification Kits and Reagents
DNA and RNA Purification Kits and Reagents
The MasterPure™ DNA Purification Kit for Blood Version II can be used to isolate exceptionally pure genomic DNA from whole blood or buffy coat. With the optimised protocol for DNA, up to 100 µg of DNA can be recovered from 600 µl of buffy coat in less than 30 minutes (Table 1). The purified DNA contains both high- and low-molecular-weight genomic DNA which can be used directly as a template in PCR amplification reactions (Figure. 1), for digestion with restriction enzymes (Figure. 2), for Southern blotting, or for other molecular biology applications. Protocols are provided for genomic DNA and TNA purification from whole blood and buffy coat. For RNA samples, we recommend the MasterPure RNA Purification Kits. Both protocols allow for scaling up to accommodate larger samples.
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| Starting Material | Yield |
| 600 µl Buffy Coat | 87-109 µg |
| 200 µl Whole Blood | 3-9 µg |
| 0.6 ml Whole Blood | 9-27 µg |
| 0.8 ml Whole Blood | 12-36 µg |
| 1 ml Whole Blood | 15-45 µg |
| 2 ml Whole Blood | 30-90 µg |
| 3 ml Whole Blood | 45-120 µg |
| 4 ml Whole Blood | 60-180 µg |
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Contact us| Europe, Middle East, and Africa | |
|---|---|
| UK | +44 1992 470 757 |
| Germany | +49 30 5304 2200 |
| North America, Latin America | |
| Wisconsin, USA | +1 888 575 9695 |
| Asia Pacific | |
| China | +8621-22509000 |
| Singapore | +65 6734 4800 |
