The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from yeast cell types (including Candida, Saccharomyces, Schizosaccharomyces) and filamentous fungi. The kit uses a rapid salting-out process¹ to remove contaminating macromolecules, avoiding toxic organic solvents, bead-beating, and spheroplasting.²

References

1. Miller, S.A. et al., (1988) Nucleic Acids Res. 16, 1215.
2. Hoffman, L.M. and Jarvis, B.W. (2004) Epicentre Forum 11(2), 4.

Figure 1. Microarray analysis of yeast RNA purified with the MasterPure™ Yeast RNA Purification Kit. The RNA was reverse transcribed, labelled, and hybridised to a S. cerevisiae DNA microarray. The cDNA from cells under oxidative stress was labelled with Cy5, and cDNA from control cells was labelled with Cy3.	Figure 2. Purity of RNA obtained using the MasterPure™ Yeast RNA Purification Kit. The RNA used in Figure. 1. was stored at -20 °C for two months before analysis on an Agilent Technologies 2100 Bioanalyzer®. The electrophoretogram demonstrates the high-purity, intact RNA.