My plate reader cannot read ROX. Can I still use KASP chemistry?
To read KASP chemistry, it is essential that your plate reader is capable of reading FAM and HEX fluorophores. It is not essential to read the ROX levels in your reactions, but it is advisable to do so as normalisation will improve the quality of the data obtained. Many of the commonly used qPCR instruments are capable of reading ROX but in some cases the software linked to the instrument does not utilise ROX in the genotyping calculations; it is still possible to obtain good quality genotyping data with these systems.