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  2. KASP-TF (Triton-Free) Master Mixes

KASP-TF (Triton-Free) Master Mixes

What you need to know about the new KASP-TF Master Mixes and how we extensively validated performance

Topics covered

  • Why we updated KASP Master Mix formulations and what changes to expect
  • How KASP-TF formulations meet and exceed our rigorous testing standards
  • Data from our comprehensive validation experiments

See the new KASP-TF Master Mixes

Why were KASP-TF Master Mixes developed?

KASP Master Mix formulations have been updated to remove Triton X-100 in compliance with the REACH Regulation for Registration, Evaluation, Authorization and Restriction of Chemicals ((EC) No 1907/2016), which was implemented by the European Commission to protect human health and the environment through the safe use of chemicals. As of January 04, 2021, use of nonylphenol ethoxylate (NPE) and octylphenol ethoxylate (OPE) based surfactants such as Triton-X100 are not permitted in the EU, unless an authorization is granted or the use is exempted from authorization. The updated KASP-TF Master Mixes are free of Triton-X100, with the same performance and quality you have always expected from your KASP genotyping mix.

Collect data for any template sequence or scale

The reformulated KASP™-TF Master Mix enables you to obtain reliable clustering and collect high-quality data you know and trust — no matter the template or scale.

We performed extensive validation testing to cover the wide range of sample types our customers study to confirm that the new formulation does not have any impact on the end-point genotyping data in comparison to the old KASP Master Mix formula.

Download application note

KASP-TF Master Mix validation in numbers

Tissue types: 6
Extraction methods: 4
Sample sets (tissue type + extraction method): 10
Genotyping platforms: 3
Unique samples: 986
Plates and arrays run: 862

Total number of data points: 585,600

Run your assay, your way

No matter what the scale, platform, DNA extraction method, species, or template sequence, our experienced scientific support empowers you to successfully develop and troubleshoot your KASP process.

In order to ensure your genotyping workflows remain uninterrupted, we are equipping you with our recommendations for setting up your comparative validation experiments.

Download quick guide

Consult our experienced scientific support team

We act as your support system, not just for the technology but also for your applications.

Please contact us with your questions.

Additional resources

KASP-TF Master Mix

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View webinar slides

Download slides

KASP Master Mix Triton-free formulations: complying with new regulations

Read the App Note

Quick guide for validating KASP Triton-free Master Mix

Download quick guide

KASP-TF Master Mix Catalog Numbers

Find your recommended KASP-TF Master Mix in the table below. You can also refer to the KASP genotyping protocols for your PCR instrument to select the correct KASP-TF Master Mix for your instrument.

Discontinued KASP Master Mixes New KASP-TF Master Mixes
Catalog Number Description Catalog number Description
KBS-1016-001 KASP V4.0 2X Master Mix 96/384, Standard ROX, 500 x 10 μL Reactions - 2.5 mL KBS-1050-101 KASP-TF V4.0 2X Master Mix 96/384, Standard ROX (2.5 mL)
KBS-1016-001-US KASP V4.0 2X Master Mix 96/384, Standard ROX, 500 x 10 μL Reactions - 2.5 mL USA/AU
KBS-1016-002 KASP V4.0 2X Master Mix 96/384, Standard ROX, 5 000 x 10 μL Reactions - 25 mL KBS-1050-102 KASP-TF V4.0 2X Master Mix 96/384, Standard ROX (25 mL)
KBS-1016-002-US KASP V4.0 2X Master Mix 96/384, Standard ROX, 5 000 x 10 μL Reactions - 25 mL USA/AU
KBS-1016-003 KASP V4.0 2X Master Mix 96/384, Standard ROX, 50 000 x 10 μL Reactions - 250 mL KBS-1050-103 KASP-TF V4.0 2X Master Mix 96/384, Standard ROX (250 mL) (10 x 25 mL)
KBS-1016-003-US KASP V4.0 2X Master Mix 96/384, Standard ROX, 50 000 x 10 μL Reactions - 250 mL - 10 x 25 mL USA/AU
KBS-1016-003B-US KASP V4.0 2X Master Mix 96/384, Standard ROX, 50 000 x 10 μL Reactions - 250 mL - 1 x 250 mL USA/AU KBS-1050-104 KASP-TF V4.0 2X Master Mix 96/384, Standard ROX (250 mL)
KBS-1016-010 KASP V4.0 2X Master Mix 1536, Standard ROX, 5 000 x 1 μL Reactions - 2.5 mL KBS-1050-111 KASP-TF V4.0 2X Master Mix 1536, Standard ROX (2.5 mL)
KBS-1016-010-US KASP V4.0 2X Master Mix 1536, Standard ROX, 5 000 x 1 μL Reactions - 2.5 mL USA/AU
KBS-1016-011 KASP V4.0 2X Master Mix 1536, Standard ROX, 50 000 x 1 μL Reactions - 25 mL KBS-1050-112 KASP-TF V4.0 2X Master Mix 1536, Standard ROX (25 mL)
KBS-1016-011-US KASP V4.0 2X Master Mix 1536, Standard ROX, 50 000 x 1 μL Reactions - 25 mL USA/AU
KBS-1016-012 KASP V4.0 2X Master Mix 1536, Standard ROX, 500 000 x 1 μL Reactions - 250 mL - 10 x 25 mL KBS-1050-113 KASP-TF V4.0 2X Master Mix 1536, Standard ROX (250 mL) (10 x 25 mL)
KBS-1016-012-US KASP V4.0 2X Master Mix 1536, Standard ROX, 50 000 x 1 μL Reactions - 250 mL - 10 x 25 mL USA/AU
KBS-1016-012B KASP V4.0 2X Master Mix 1536, Standard ROX, 500 000 x 1 μL Reactions - 250 mL - 1 x 250 mL KBS-1050-115 KASP-TF V4.0 2X Master Mix 1536, Standard ROX (250 mL)
KBS-1016-012B-US KASP V4.0 2X Master Mix 1536, Standard ROX, 500 000 x 1 μL Reactions - 250 mL - 1 x 250 mL USA/AU
KBS-1016-016 KASP V4.0 2X Master Mix 96/384, Low ROX, 500 x 10 μL Reactions - 2.5 mL KBS-1050-121 KASP-TF V4.0 2X Master Mix 96/384, Low ROX (2.5 mL)
KBS-1016-016-US KASP V4.0 2X Master Mix 96/384, Low ROX, 500 x 10 μL Reactions - 2.5 mL USA/AU
KBS-1016-017 KASP V4.0 2X Master Mix 96/384, Low ROX, 5 000 x 10 μL Reactions - 25 mL KBS-1050-122 KASP-TF V4.0 2X Master Mix 96/384, Low ROX (25 mL)
KBS-1016-017-US KASP V4.0 2X Master Mix 96/384, Low ROX, 5 000 x 10 μL Reactions - 25 mL USA/AU
KBS-1016-018 KASP V4.0 2X Master Mix 96/384, Low ROX, 50 000 x 10 μL Reactions - 250 mL KBS-1050-123 KASP-TF V4.0 2X Master Mix 96/384, Low ROX (250 mL) (10 x 25 mL)
KBS-1016-018-US KASP V4.0 2X Master Mix 96/384, Low ROX, 50 000 x 10 μL Reactions - 250 mL - 10 x 25 mL USA/AU
KBS-1016-018B-US KASP V4.0 2X Master Mix 96/384, Low ROX, 50 000 x 10 μL Reactions - 250 mL - 1 x 250 mL USA/AU KBS-1050-124 KASP-TF V4.0 2X Master Mix 96/384, Low ROX (250 mL)
KBS-1016-021 KASP V4.0 2X Master Mix 96/384, High ROX, 500 x 10 μL Reactions - 2.5 mL KBS-1050-131 KASP-TF V4.0 2X Master Mix 96/384, High ROX (2.5 mL)
KBS-1016-021-US KASP V4.0 2X Master Mix 96/384, High ROX, 500 x 10 μL Reactions - 2.5 mL USA/AU
KBS-1016-022 KASP V4.0 2X Master Mix 96/384, High ROX, 5 000 x 10 μL Reactions - 25 mL KBS-1050-132 KASP-TF V4.0 2X Master Mix 96/384, High ROX (25 mL)
KBS-1016-022-US KASP V4.0 2X Master Mix 96/384, High ROX, 5 000 x 10 μL Reactions - 25 mL USA/AU
KBS-1016-023 KASP V4.0 2X Master Mix 96/384, High ROX, 50 000 x 10 μL Reactions - 250 mL KBS-1050-133 KASP-TF V4.0 2X Master Mix 96/384, High ROX (250 mL) (10 x 25 mL)
KBS-1016-023-US KASP V4.0 2X Master Mix 96/384, High ROX, 50 000 x 10 μL Reactions - 250 mL USA/AU
KBS-1030-001 KASP Array Tape 2X Master Mix, Standard ROX, - 2.5 mL KBS-1050-201 KASP-TF V5.0 2X Master Mix, Standard ROX (2.5 mL)
KBS-1030-002 KASP Array Tape 2X Master Mix, Standard ROX, - 25 mL KBS-1050-202 KASP-TF V5.0 2X Master Mix, Standard ROX (25 mL)
KBS-1030-004 KASP Array Tape 2X Master Mix, Standard ROX, - 250 mL (10 x 25 mL) KBS-1050-203 KASP-TF V5.0 2X Master Mix, Standard ROX (250 mL) (10 x 25 mL)
KBS-1030-003 KASP Array Tape 2X Master Mix, Standard ROX, - 250 mL KBS-1050-204 KASP-TF V5.0 2X Master Mix, Standard ROX (250 mL)