Useful phosphoramidite for modification of base pairing and metal chelating characterisitcs of purine nucleosides through elimination of the N7 nitrogen.
Isosteric with adenine but offers a different pi-electron distribution and thus an altered dipole moment.
A stable phosphoramidite analogue of N3-methyladenine (3-Me-A).
FAM-AYC ACA TTG GCA CCC GCA ATC CTG-BHQ1
FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1
FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1
GAC CCC AAA ATC AGC GAA AT
TCT GGT TAC TGC CAG TTG AAT CTG
GGG AGC CTT GAA TAC ACC AAA A
TGT AGC ACG ATT GCA GCA TTG
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