CPG for incorporation of unmodified dG at 3' end of an oligonucleotide.
This phosphoramidite installs a thymidine residue bearing a bulky NPOM protecting group at N1, thus efficiently disrupting Watson-Crick base-pairing.
After incorporation into an oligonucleotide, irradiation at 365 nm causes strand cleavage, leaving a phosphate on the 5' end and a hydroxyl residue on the 3' end.
Digest unwanted contaminating linear double-stranded DNA, without harming circular double-stranded DNA such as plasmids and fosmids.
After incorporation into an oligonucleotide, irradiation at 365 nm at neutral pH causes strand cleavage, leaving phosphate groups on both daughter strands.
CPG used to incorporate a deoxyinosine base into the 3' end of an oligonucleotide.
Phosphoramidite used to incorporate a dA nucleobase when creating phosphorodithioate (PS2) linkages.
CPG useful for synthesising 2'-5' linked oligonucleotides, and effecting 3' termination.
1/16" OD x 0.020" ID x 24", pre-cut Teflon tubing with Upchurch fittings.
1/16" OD x 0.020" ID x 48 ", pre-cut Teflon tubing with Lee valve fittings.
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