BlackBerry Quenchers

BlackBerry™ Quencher 650 (BBQ-650™) is ideal for the quenching of long-wavelength reporter fluorophores, useful in various probe applications involving contact or FRET quenching.

Non-fluorescent, absorbs light at long wavelengths

• Useful absorbance between 550 and 750 nm
• λ_max varies from 650 to >690 nm in various oligonucleotides
• Quenches long-wavelength fluorophores such as Cy3, TAMRA, Texas Red, Cy5, and Cy5.5

Robust, stable to synthesis

• Ammonia stable for mild deprotection.
• Install at internal, 3', or 5' positions with standard solid-phase oligonucleotide synthesis.

Excellent contact-mode quencher

• Highly efficient quencher of FAM, Cy3, Texas Red, Cy5, and Cy 5.5 in contact quenching applications such as molecular beacons.
• Particularly high affinity for Cy dyes. Signal-to-background ratios of >90 and >88 for Cy5 and Cy5.5, respectively, were observed in molecular beacons.

Excellent FRET-mode quencher

• Excellent spectral overlap with long-wavelength fluorophores such as Texas Red, ROX, Cy5, and Cy 5.5, thus allowing efficient FRET quenching.
• Cy5.5 was quenched with 99% efficiency when separated from BBQ-650™ by ten base pairs (ca. 40 Å) in a non-contact hybrid probe.